Department of Ophthalmology

Peer-Reviewed Publications 2009-2014

Takahashi Y, Moiseyev G, Ablonczy Z, Chen Y, Crouch RK and Ma JX. Identification of a novel palmitylation site essential for membrane association and isomerohydrolase activity of RPE65. J Biol Chem 284:3211-3218, 2009, PMID: 19049981, PMCID: PMC2631947.

Parker RO, Fan J, Nickerson JM, Liou GI, and Crouch RK. Normal cone function requires the interphoto-receptor retinoid binding protein. J Neurosci 29:4616-4621, 2009, PMID: 19357286, NIHMSID: NIHMS107856.

Park PS, Sapra KT, Jastrzebska B, Maeda T, Maeda A, Pulawski W, Kono M, Lem J, Crouch RK, Filipek S, Müller DJ, and Palczewski K. Modulation of molecular interactions and function by rhodopsin palmitylation. Biochemistry 48:4294-4304, 2009, PMID: 19348429, PMCID: PMC2710298.

Isayama T, McCabe-England SL, Crouch RK, Zimmerman AL, and Makino CL. β-ionone activates and bleaches visual pigment in salamander photoreceptors. Vis Neurosci 26:267-274, 2009, PMID: 19500430, PMCID: PMC3220273.

Ala-Laurila P, Cornwall MC, Crouch RK, and Kono M. The action of 11-cis-retinol on cone opsins and intact cone photoreceptors. J Biol Chem 284:16492-16500, 2009, PMID: 19386593, PMCID: PMC2713570.

Estevez ME, Kolesnikov AV, Ala-Laurila P, Crouch RK, Govardovskii VI, and Cornwall MC. The 9-methyl group of retinal is essential for rapid Meta II decay and phototransduction quenching in red cones. J Gen Physiol 134:137-150, 2009, PMID: 19635855, PMCID: PMC2717693.

Kunchithapautham K, Coughlin B, Crouch RK, and Rohrer B. Cone outer segment morphology and cone function in the Rpe65-/- Nrl-/- mouse retina are amenable to retinoid replacement. Invest Ophthalmol Vis Sci 50:4858-4864, 2009, PMID: 19407011.

Parker RO, and Crouch RK. The visual cycle: Generation of 11-cis retinal for photoreceptors. Photobiol Online <>, 2009.

Farjo KM, Moiseyev G, Takahashi Y, Crouch RK, and Ma JX. RDH10 has 11-cis-retinol dehydrogenase activity and Interacts with visual cycle proteins. Invest Ophthalmol Vis Sci 50:5089-5097 (figure selected for cover), 2009, PMID: 19458327.

Gonzalez-Fernandez F, Bevilacqua T, Lee KI, Chandrashekar R, Hsu L, Garlipp MA, Griswold JB, Crouch RK, and Ghosh D. Retinol-binding site in interphotoreceptor retinoid-binding protein (IRBP): A novel hydrophobic cavity. Invest Ophthalmol Vis Sci 50:5577-5586, 2009, PMID: 19608538.

Parker RO, McCarragher B, Crouch RK, and Darden AG. Photoreceptor development in premetamorphic and metamorphic Xenopus laevis. Anat Rec 293:383-387, 2010, PMID: 20091886.

Fan J, Sakurai K, Chen C-K, Rohrer B, Wu BX, Yau K-W, Kefalov V, and Crouch RK. Deletion of GRK1 causes retina degeneration through a transducin-independent mechanism. J Neurosci 30:2496-2503, 2010, PMID: 20164334, PMCID: PMC2849294.

Parker RO, and Crouch RK. The interphotoreceptor retinoid binding (IRBP) is essential for normal retinoid processing in cone photoreceptors. Adv Exp Med Biol 664:141-149, 2010, PMID: 20238012.

Best CL, Smith DW, Raymond JR, Greenberg RS, and Crouch RK. Preventing and responding to complaints of sexual harassment in an academic health center: A 10-year review from the Medical University of South Carolina. Acad Med 85:721-727, 2010, PMID: 20354396.

Maeda A, Okano K, Park PS, Lem J, Crouch RK, Maeda T, and Palczewski K. Palmitoylation stabilizes unliganded rod opsin. Proc Natl Acad Sci USA 107:8428-8433, 2010, PMID: 20404157, PMCID: PMC22889565.

Kono M, and Crouch RK. In vitro assays of rod and cone opsin activity: Retinoid analogs as agonists and inverse agonists. Methods Mol Biol 652:85-94, 2010, PMID: 20552423.

Kolesnikov AV, Fan J, Crouch RK, and Kefalov V. Age-related deterioration of rod vision in mice. J Neurosci. 30:11222-11231, 2010, PMID: 20720130, PMCID: PMC2928554.

Makino CL, Riley CK, Looney J, Crouch RK, and Okada T. Binding of more than one retinoid to visual opsins. Biophys J. 99:2366-2373, 2010, PMID: 20923672, PMCID: PMC3042582.

Gutierrez DB, Blakeley L, Goletz PW, Schey KL, Hanneken A, Koutalos Y, Crouch RK, and Ablonczy Z. Mass spectrometry provides accurate and sensitive quantitation of A2E. Photochem Photobiol Sci 9:1513-1519, 2010, PMID: 20931136, PMCID: PMC23013054.

Tang PH, Fan J, Goletz PW, Wheless L, and Crouch RK. Effective and sustained delivery of hydrophobic retinoids to photoreceptors. Invest Ophthalmol Vis Sci 51:5958-5964, 2010, PMID: 20574023, PMCID: PMC3061517.

Parker RO, and Crouch RK. Retinol dehydrogenases (RDHs) in the visual cycle. Exp Eye Res. 91:788-792, 2010, PMID: 20801113, PMCID: PMC3065351.

Parker R, Wang JS, Kefalov VJ, and Crouch RK. Interphotoreceptor retinoid-binding protein as the physiologically relevant carrier of 11-cis-retinol in the cone visual cycle. J Neurosci 31:4714-4719, 2011, PMID: 21430170, PMCID: PMC3226706

Kono M, and Crouch RK. Probing human red cone opsin activity with retinal analogues. J Nat Prod 74:391-394, 2011, PMID: 21314100, PMCID: PMC3064742.

Fan J, Crouch RK, and Kono M. Light prevents exogenous 11-cis retinal from maintaining cone photoreceptors in chromophore-deficient mice. Invest Ophthalmol Vis Sci 52:2412-2416, 2011, PMID: 21228385, PMCID: PMC3081252.

Kolesnikov AV, Tang PH, Parker RO, Crouch RK, and Kefalov VJ. The mammalian cone visual cycle promotes rapid m/l-cone pigment regeneration independently of the interphotoreceptor retinoid-binding protein. J Neurosci 31:7900-7909, 2011, PMID: 21613504, PMCID: PMC3108150.

Blakeley LR, Chen C, Chen CK, Chen J, Crouch RK, Travis GH, and Koutalos Y. Rod outer segment retinol formation is independent of Abca4, arrestin, rhodopsin kinase, and rhodopsin palmitylation. Invest Ophthalmol Vis Sci 52:3483-3491, 2011, PMID: 21398289, PMCID: PMC3109038.

Grey AC, Crouch RK, Koutalos Y, Schey KI, and Ablonczy Z. Spacial localization of A2E in the retinal pigment epithelium. Invest Ophthalmol Vis Sci 52:3926-3933, 2011, PMID: 21357388, PMCID: PMC3175942.

Tang PH, Wheless L, and Crouch RK. Regeneration of photopigment is enhanced in mouse cone photoreceptors expressing RPE65 protein. J Neurosci 31:10403-10411, 2011, PMID: 21753017, PMCID: PMC3153911.

Tang PH, Buhusi MC, Ma JX, and Crouch RK. RPE65 is present in human green/red cones and promotes photopigment regeneration in an in vitro cone cell model. J Neurosci 31:18618-18626, 2011, PMID: 22171060.

Ablonczy Z, Gutierrez DB, Grey AC, Schey KL, and Crouch RK. Molecule-specific imaging and quantitation of A2E in the RPE. Adv Exp Med Biol 723:75-81, 2012, PMID: 22183318.

Frederiksen R, Crouch RK, Nichol B, Chakrabarti KS, Oprian D, Koutalos Y, Boyer NP, and Cornwall MC. Increased rate of pigment regeneration and dark adaptation in rod photoreceptors containing 11-cis 4-OH rhodopsin. J Gen Physiol, 2012

Boyer NP, Tang PH, Higbee D, Ablonczy Z, Crouch RK, and Koutalos Y. Lipofuscin and A2E Accumulate with Age in the Retinal Pigment Epithelium of Nrl(-/-) Mice(†). Photochem Photobiol, 2012, PMID: 22417141

Frederiksen R, Boyer NP, Nickle B, Chakrabarti KS, Koutalos Y, Crouch RK, Oprian D, and Cornwall MC. Low aqueous solubility of 11-cis-retinal limits the rate of pigment formation and dark adaptation in salamander rods. J Gen Physiol. 139:493-505, 2012, PMID: 22641642

Boyer NP, Higbee D, Currin MB, Blakeley LR, Chen C, Ablonczy Z, Crouch RK, and Koutalos Y. Lipofuscin and N-retinylidene-N-retinylethanolamine (A2E) accumulate in the retinal pigment epithelium in the absence of light exposure: Their origin is 11-Cis retinal. J Biol Chem. 2012, PMID: 22570475

Tang PH, Kono M, Koutalos Y, Ablonczy Z, Crouch RK: New insights into retinoid metabolism and cycling within the retina: Prog Retin Eye Res. 32:48-63: 2013, PMID: 23063666, PMCID: PMC3746031

Ablonczy Z, Higbee D, Anderson DM, Dahrouj M, Grey AC, Gutierrez D, Koutalos Y, Schey KL, Hanneken A, Crouch RK: Lack of correlation between the spatial distribution of A2E and lipofuscin fluorescence in the human retinal pigment epithelium: Invest Ophthalmol Vis Sci. 54(8):5535-42, 2013, PMID: 23847313, PMCID: PMC3747789

Ablonczy Z, Higbee D, Grey AC, Koutalos Y, Schey KL, Crouch RK: Similar molecules spatially correlate with lipofuscin and N-retinylidene-N-retinylethanolamine in the mouse but not in the human retinal pigment epithelium:  Arch Biochem Biophys. 539(2):196-202, 2013, PMID: 23969078, PMCID: PMC3818512

Isayama T, Chen Y, Kono M, Fabre E, Slavsky M, Degrip WJ, Ma JX, Crouch RK, Makino CL (2014) Coexpression of three opsins in cone photoreceptors of the salamander Ambystoma tigrinum. J Comp Neurol. Jul 1;522:2249-65, 2014., PMID: 24374736

Ablonczy Z, Smith N, Anderson DM, Grey AC, Spraggins J, Koutalos Y, Schey KL, Crouch RK:  The utilization of fluorescence to identify the components of lipofuscin by imaging mass spectrometry:  Proteomics:  14:936-944, 2014., PMID: 24453194

Anderson DMG, Ablonczy Z, Koutalos Y, Spraggins J, Crouch RK, Caprioli RM, Schey KL. (2014). High resolution 10μm MALDI imaging mass spectrometry of retinal tissue lipids. J Am Soc Mass Spectrom, (in press).    

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